| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walker, Lucy E (15)
|
MRBGNW |
25.26 |
24.95 |
-0.31 |
| 2 |
Lee, Megan C (17)
|
MRWEST |
24.40 |
25.21 |
0.81 |
| 3 |
Sohn, Sora (17)
|
MRBGNW |
26.11 |
25.63 |
-0.48 |
| 4 |
Zorgman, Sunell (15)
|
MRBGNW |
24.93 |
25.95 |
1.02 |
| 5 |
Kung, Annabelle D (15)
|
MRBGNW |
25.91 |
26.34 |
0.43 |
| 6 |
Rocco, Emily A (17)
|
MRBGNW |
25.40 |
26.39 |
0.99 |
| 7 |
McIntyre, Savannah Y (16)
|
MRBGNW |
25.64 |
26.80 |
1.16 |
| 8 |
Blakeslee, Kaitlin P (17)
|
MRBGNW |
26.26 |
27.34 |
1.08 |
| 9 |
Storch, Rebecca H (15)
|
MRPAC |
26.29 |
27.79 |
1.50 |
| 10 |
Desiderio, Sarah P (17)
|
MRBGNW |
27.14 |
28.34 |
1.20 |
| 11 |
Abrams, Anna M (17)
|
MRPAC |
26.99 |
29.29 |
2.30 |